Construction and characterization of a dunaliella viridis bacterial artificial chromosome library 細菌人工染色體文庫的構建和鑒定
In order to construct plant artificial chromosome , plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms 為了構建植物人工染色體,我們構建了可以和含有著絲粒序列的yac載體的左臂和右臂進行同源重組的質粒載體。
The successfully constructed artificial chromosome would also can be used as a new vector to introduce chromosome segments of hundreds kb to mb length into plant cells 成功構建的植物人工染色體也可以作為一種新型的載體用來克隆和轉化長達幾百kb到一個mb的外源dna進入植物細胞。
To research melanin synthesis and its accumulation mechanism , we constructed a silky bacterial artificial chromosome ( bac ) library . the library was used to study the two genes on chicken chr 為研究絲羽烏骨雞體內黑色素合成和沉積的分子機制,本研究構建了絲羽烏骨雞的基因組細菌人工染色體( bac )文庫。
The construction and introduction of plant artificial chromosomes into plant cells would be an effective way to understand the essential elements necessary for maintaining chromosome function and structure in plants 1997年,美國的casewesternreserve宣布構建成功第一代hac 。在植物細胞中構建人工染色體將為植物染色體的結構和功能以及染色體的各必須組分的研究提供有效的工具和手段。
It have been reported that the 210 kb yeast artificial chromosome ( yac ) including the human a - lactalbumin gene could direct the transgene to express in a position - independent manner , therefore is an excellent expression vector to produce transgenic animal bioreactors 根據報道含完整人-乳白蛋白基因的yac載體能指導外源基因的乳腺特異性高效表達,是生產動物乳腺生物反應器的理想表達載體。
Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis . the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method 用脈沖電泳將經過同源重組的修飾的yac克隆分離出來通過脂質體轉化植物的原生質體,希望得到在植物細胞中穩定存在的植物人工染色體。
The optimum parameters , such as the exact time for the activated eggs finishing the first chromosome sets copying , the optimum period for artificial chromosome set duplication , the optimum temperature and intensity of heat shocking etc , were determined 確定了在一定孵化溫度下,被激活的草魚卵子完成第一次染色體復制的準確時間,實施人工染色體加倍處理的最佳時機,人工加倍處理的最佳方法和處理強度等。